Exactive quickstart guide thermo fisher scientific. The q exactive hfx mass spectrometer uses a highcapacity transfer tube for maximum ion loading, an electrodynamic ion funnel that accommodates and transmits ions over a broader mass range, and a highfield thermo scientific tm orbitrap tm mass analyzer. Here we describe the q exactive plus and q exactive hf. The q exactive hf system combines a stateoftheart segmented quadrupole for highperformance precursor ion selection with a highresolution, accuratemass hram ultrahighfield orbitrap mass analyzer to deliver a superior combination of scan speed, resolving power, mass accuracy, spectral quality and sensitivity. The q exactive hf x system combines a stateoftheart segmented quadrupole and ultrahighfield orbitrap mass analyzer to achieve highperformance precursor ion selection with a highresolution, accuratemass hram to deliver a superior combination of scan speed, resolving power, mass accuracy, spectral quality and sensitivity. This text is a guide to operating the q exactive mass spectroscopy.
Mass spectrometers available to be used within routine core facility analyses. Refer to the q exactive operating manual for instructions about preparing the positive ion mode calibration solution or buying the readytouse solution from thermo fisher scientific. Thermo scientific q exactive hf biopharm from 2017 condition. Seppak tc18 cartridges according to the manufacturers instructions. Thermo scientific q exactive plus orbitrap lcmsms system characterize, quantify and confirm with unmatched confidence the thermo scientific q exactive plus benchtop lcmsms combines highperformance quadrupole precursor selection with highresolution, accuratemass hram orbitrap detection to deliver. A q exactive hf mass spectrometer equipped with hesiii ion source was employed. Thermo scientific q exactive plus orbitrap lcmsms system. A more detailed and thorough account of these procedures can be found in the q exactive operating manual. An advanced active beam guide increases ion transmission. Detection of 1 zmol injection of angiotensin using. Performance evaluation of the q exactive hfx for shotgun. To view product manuals go to start programs thermo exactive manuals. Vacuum system thermo fisher scientific exactive operating manual 5. Q exactive hf x is the most recently developed ms instrument in the benchtop orbitrap series, with a novel peak selection algorithm and a bright ion.
Q exactive hybrid quadrupoleorbitrap mass spectrometer. The q exactive hf system thermo scientific is a hybridquadrupoleorbitrap mass spectrometer, which provides highresolution mass spectra with high mass accuracy. Thermo scientific q exactive hf orbitrap lcmsms system higherquality data, faster than ever the thermo scientific q exactive hf benchtop lcmsms combines a highresolution accuratemass hram ultrahighfield orbitrap analyzer and a high performance quadrupole precursor ion selection to deliver unsurpassed speed while. The q exactive hf instrument uses a segmented quadrupole mass filter to achieve more precise precursor isolation for better discrimination between analytes and coeluting interferences. Refer to the q exactive operating manual for instructions about preparing the positive ion mode calibration solution or buying the readytouse solution from thermo fisher scientific product number 88323. Anything i can do, aside from avoiding them, to decrease the problem. Refurbished thermo scientific q exactive hybrid quadrupoleorbitrap mass spectrometer.
Please note that this guide does not contain any safety notices and precautionary statements. After purchase you will receive a download link together with a license key that is needed to activate. Dataindependent acquisition for the orbitrap q exactive hf. How much orbitrap q exactive plus costs compared to. Q exactive classicplus instruments were not tested and are not officially supported. This is how to set up a q exactive for intact protein analysis by ben orsburn on vimeo, the home for high quality videos and the people who love them. Thermo fisher q exactive mass spectrometer hf hf x.
Refer to the exactive series operating manual for instructions about preparing the positiveionmode calibration solution or buying the readytouse solution from. Here we describe the q exactive plus and q exactive hf mass. This guide is intended for all personnel who use to operate the exactive. Im working on the project for protein identification using. The q exactive hf x also enables rapid phosphoproteomics with routine analysis of more than 5000 phosphopeptides with short singleshot 15 min lcmsms measurements, or 16 700 phosphopeptides quantified across ten conditions in six gradient hours using tmt10plex and offline peptide fractionation.
The result is superior sensitivity and greater quantitative dynamic range. Average data dependent scan speed of 1618 hz over the whole gradient is obtained on the q exactive hf ms using a hela proteolytic digest. The application is realized with stateoftheart lcms instrumentations. A more detailed and thorough account of these procedures can be found in the qexactive operating manual. The vent valve closes after power is restored to the instrument.
About this guide this q exactive hf software manual provides reference information about the parameters in the instrument configuration window, the instrument setup window, and the q exactive hf tune window. Q exactive hf is a member of the thermo scientific family of mass spectrometer ms detectors that are powered by orbitrap technology. When power to the vent valve solenoid is shut off, the vent valve opens and the manifold is vented with nitrogen. The q exactive hf benchtop orbitrap mass spectrometer shows excellent performance for the analysis of complex proteolytic protein digests, analyzed in 30, 60 and 120 min run times. The q exactive hf, a benchtop mass spectrometer with a prefilter.
The q exactive hf system combines a stateoftheart segmented quadrupole for highperformance precursor ion selection with a highresolution, accuratemass hram ultrahighfield orbitrap mass analyzer to deliver a. The q exactive hf mass spectrometer supports multiple approaches to quantitation including selected ion monitoring sim, parallelreaction monitoring prm, and dataindependent acquisition dia. Dataindependent acquisition for the orbitrap q exactive. Refer to the exactive series operating manual for instructions about preparing the positive ion mode calibration solution or buying the readytouse solution from. See figure 115 on page 128 and figure 119 on page 7. The thermo q exactive orbitrap is a high resolution mass spectrometer suitable for accurate mass measurement as well as msms studies. This webinar, in two parts, will cover the development of an optimized data independent acquisition dia workflow for a proteomics study using the thermo scientific q exactive hf. Advanced quadrupole design for very stable system operation and. The q exactive hf system combines a stateoftheart segmented quadrupole for highperformance precursor ion selection with a highresolution, accuratemass hr am ultrahighfield orbitrap mass analyzer to deliver a superior combination of scan speed, resolving power, mass accuracy, spectral quality and sensitivity. There are several ways to contact thermo fisher scientific. This exactive quickstart guide provides an overview on how to set up and use the thermo scientific exactive ms detector. It is capable of ionization using conventional heated electrospray hesi, atmospheric pressure chemical ionization apci, and nanoflow electrospray nanoesi. An engineer cleans the instrument so you are saying the in gel preps are the problem.
The q exactive mass spectrometer is equally useful for untargeted or targeted screening and a broad range of qualitative and quantitative applications in drug discovery, proteomics, environmental and food safety, clinical research and forensic toxicology. Thermo fisher scientifics analytical instruments receive. Using the q exactive hf x hybrid, we have managed to identify 1,100 unique peptides per minute, which is a new world record. Manuals for the delivered ion sources and other software. This q exactive hf has been modified in house with a unique quadrupole and quadrupole electronics control for the analysis of higher mz ranges compared to typical qe hf instruments. Characterization and tentative identification of new. Amensonlamar, emily ann, sun, liangliang, zhang, zhenbin, bohn, paul w. A basic interpretation of this plot is that full ms is 34 ms faster on the q exactive hf x compared to the q exactive hf. Detection of 1 zmol injection of angiotensin using capillary zone electrophoresis coupled to a q exactive hf mass spectrometer with an electrokinetically pumped sheathflow electrospray interface author. Exactive series operating manual revision a thermo fisher. Q exactive hf software manual thermo fisher scientific. The superior quality of q exactive plus msms data enables identification and quantitation of more compounds with greater confidence. Thermo q exactive hf from 2017, biopharm, in excellent.
It presents suggestions based on sample complexity and number of peptides to quantify. Q exactive hf hybrid quadrupoleorbitrap mass spectrometer. Exactive operating manual thermo fisher scientific. Thermo q exactive hf from 2017, biopharm, in excellent condition. You can access pdf files of the documents listed above from the data system computer. Discovering potential diabetic lipid biomarkers using hram. Thermo fisher q exactive of enabling technologies has superior resolution and high sensitivity which allows quantitative. The thermo scientific vanquish uhplc system is coupled to the thermo scientific q exactive hf hybrid quadrupoleorbitrap mass spectrometer. The q exactive hf mass spectrometer features an ultrahigh field orbitrap analyzer which doubles its speed and resolution compared to the first generation of orbitrap analyzer figure 1. Together, these components enable fast identification and analysis of peptides, labelfree and tmt quantitation, topdown proteomics analyses, sophisticated dda and dia, drtprm and biopharma characterization. A qexactive plus mass spectrometer coupled with the easy nano uplc system, working in concert with our existing bioinformatics pipeline, will play a vital role in advancing projects tha span a broad range of fundamental developmental processes and applied studies cancer, bacterial infections. How to set up a q exactive for intact protein analysis on.
The q exactive hfx ms takes identification and quantitation to. Exactive operating manual manuals for the delivered ion sources and other software. The q exactive hf x ms can identify the same amount of proteins as older q exactive mass spec in half the time. Refer to your lc manual for the heat output of your lc equipment. With a fast scan speed and multiplexing capabilities, the q exactive plus mass spectrometer is an outstanding detector for fast chromatography separation techniques. Velos velos pro velos q exactive hf q exactive plus q exactive q exactive fetipl 7000520606 0. Exactive plus operating manual pn 23060, revision a i. Together, these components enable fast identification and analysis of peptides, labelfree and tmt.
Using the same gradient, the hf x is able to identify 40% more peptides than the q exactive hf ms and 95% more than the q exactive ms. The q exactive hf is coupled to two lc systems, the vanquish horizon binary uhplc and the dionex integrion rfic thermo scientific. The air conditioning load for a typi cal q exactive lcms system with data system and a ty pical lc is approximat ely 3. Thermo fisher will showcase the new q exactive hf x spectrometer as part of a workflow that includes advanced instrumentation, columns and software to support customers from sample to knowledge. Please contact us for complete refurbishedused thermo q exactive mass spectrometer system, warranty and service contract details. Using it of between 75 to 120 ms for ms2 should i decrease this to 50. This is one of our most versatile instruments, which is used as a denaturing topdown instrument with the standard quadrupole electronics board and a dionex. Table 31 shows the approximate heat output of each module. Characterization and tentative identification of new flunitrazepam metabolites in authentic human urine specimens using liquid chromatography. Thermo scientific q exactive hf orbitrap lcmsms system. How much orbitrap q exactive plus costs compared to orbitrap exactive plus. Improvements in shotgun proteomics using a benchtop. Users can select the technique best matched to their sample and information requirements. How to realize lcms quantitation with chromeleon 7.
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